Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23578

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates and tissue homogenates (50 - 100 uL)

Sensitivity

0.98 ng/mL

Assay Range

3.9 ng/mL - 250 ng/mL

View all ST6 Gal Sialyltransferase 1/ST6GAL1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery

Recovery

80 - 103%

Scientific Data Images

Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 ELISA Kit (Colorimetric)

Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 ELISA Kit (Colorimetric) [NBP3-23578] -

Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 ELISA Kit (Colorimetric) [NBP3-23578] - Standard Curve Reference

Kit Contents

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin - antibody (100 x concentrate)
  • Biotin - antibody Diluent
  • HRP - avidin (100 x concentrate)
  • HRP - avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

CD75 is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. This antibody recognizes the CD75 cell surface antigen, an alpha 2,6 sialyated carbohydrate epitope expressed by mature B cells, especially germinal centre B cells, red blood cells and some epithelial cells.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Gene Symbol

ST6GAL1

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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