Rat STAT5b ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80282

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL

View all STAT5b ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat STAT5B in samples. No significant cross-reactivity or interference between Rat STAT5B and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.25%

Inter-Assay Precision (Precision between assays) CV% < 4.83%

Recovery for Rat STAT5b ELISA Kit (Colorimetric)

Recovery

90-111%

Linearity

Rat STAT5b ELISA Kit (Colorimetric)

Scientific Data Images for Rat STAT5b ELISA Kit (Colorimetric)

Rat STAT5b ELISA Kit (Colorimetric)

ELISA: Rat STAT5b ELISA Kit (Colorimetric) [NBP2-80282] -

ELISA: Rat STAT5b ELISA Kit (Colorimetric) [NBP2-80282] - Standard Curve Reference

Kit Contents for Rat STAT5b ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: STAT5b

STAT5B is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B

Gene Symbol

STAT5B

Additional STAT5b Products

Product Documents for Rat STAT5b ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat STAT5b ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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