Human SYK - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human SYK - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40105

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40105

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.244 ng/mL (example only; lot dependent)

Assay Range

0.625 - 40 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human SYK - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human SYK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40105]

ELISA: Human SYK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40105] - Standard Curve Reference

Kit Contents for Human SYK - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SYK

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Long Name

Spleen Tyrosine Kinase

Alternate Names

DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK

Gene Symbol

SYK

Additional SYK Products

Product Documents for Human SYK - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Human SYK - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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