Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA3851

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Buffered solution, Supernatant, Plasma, Serum (100 uL)

Sensitivity

4.94 ng/mL

Assay Range

8 to 256 ng/mL

View all Syndecan-1/CD138 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. SDC1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SDC1.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.2%

Inter-Assay Precision (Precision between assays) CV% < 6.2%

Recovery for Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

Recovery

101%

Scientific Data Images for Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

ELISA: Human Syndecan-1/CD138 ELISA Kit (Colorimetric) [KA3851]

ELISA: Human Syndecan-1/CD138 ELISA Kit (Colorimetric) [KA3851]

ELISA: Syndecan-1/CD138 ELISA Kit [Biotin] [KA3851] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

  • 96 well microtitre strip plate
  • Biotinylated anti-CD138
  • Biotinylated Antibody Diluent
  • Control
  • H2SO4 stop reagent
  • HRP Diluent
  • Plastic plate covers
  • Standard Diluent (Buffer)
  • Standard: 256 ng/mL
  • Streptavidin-HRP
  • TMB Substrate
  • Wash Buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Syndecan-1/CD138 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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