Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40229

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40229

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

6.0 pg/mL (example only; lot dependent)

Assay Range

15.6 - 1000 pg/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40229]

ELISA: Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40229] - Standard Curve Reference

Kit Contents for Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Thymidine Kinase 1

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Alternate Names

TK1

Gene Symbol

TK1

Additional Thymidine Kinase 1 Products

Product Documents for Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Thymidine Kinase 1 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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