Human TIMP-1 ELISA Kit (Colorimetric)

Name: Human TIMP-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-60165

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60165

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.434 ng/mL

Assay Range

0.39 ng/mL - 25 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human TIMP-1. No significant cross-reactivity or interference between human TIMP-1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human TIMP-1 ELISA Kit (Colorimetric)

ELISA: Human TIMP-1 ELISA Kit (Colorimetric) [NBP2-60165]

ELISA: Human TIMP-1 ELISA Kit (Colorimetric) [NBP2-60165] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Human TIMP-1 ELISA Kit (Colorimetric) [NBP2-60165] -

Human TIMP-1 ELISA Kit (Colorimetric) [NBP2-60165] - Standard Curve Reference

Kit Contents for Human TIMP-1 ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Assay plate (12 x 8 coated Microwells)
Biotin-antibody (100 x concentrate)
Biotin-antibody Diluent
HRP-avidin (100 x concentrate)
HRP-avidin Diluent
Instruction manual
Sample Diluent
Standard (Freeze dried)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TIMP-1

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Gene Symbol