Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60129

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

1.6 pg/mL

Assay Range

7.8125 pg/mL - 500 pg/mL

View all TNF RII/TNFRSF1B ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse TNFsR- II . No significant cross-reactivity or interference between mouse TNFsR-II and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

ELISA: Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129]

ELISA: Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129]

ELISA: Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129] -

Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129] - Standard Curve Reference

Kit Contents for Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TNF RII/TNFRSF1B

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFsR- II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFsR-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFsR-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFsR-II bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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