Rat Transferrin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1847

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (25 uL)

Sensitivity

0.1 ug/mL

Assay Range

0.156 to 5 ug/mL

View all Transferrin ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Description

Tf (Rat) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of rat Tf.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.5%

Inter-Assay Precision (Precision between assays) CV% < 7%

Recovery for Rat Transferrin ELISA Kit (Colorimetric)

Recovery

95%

Scientific Data Images for Rat Transferrin ELISA Kit (Colorimetric)

ELISA: Rat Transferrin ELISA Kit (Colorimetric) [KA1847]

ELISA: Rat Transferrin ELISA Kit (Colorimetric) [KA1847]

ELISA: Rat Transferrin ELISA Kit (Colorimetric) [KA1847] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Rat Transferrin ELISA Kit (Colorimetric)

  • Biotinylated Transferrin: lyophilized.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Rat Transferrin Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against rat Transferrin.
  • Rat Transferrin Standard: Rat Transferrin in a buffered protein base, lyophilized.
  • Sealing Tapes: pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydroxychloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Transferrin

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Rat Transferrin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Transferrin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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