Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)

Name: Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38698

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38698

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

3.27 ng/mL (example only; lot dependent)

Assay Range

6.25 - 400 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)

ELISA: Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric) [NBP3-38698]

ELISA: Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric) [NBP3-38698] - Standard Curve Reference

Kit Contents for Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents for Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Transgelin/TAGLN/SM22 alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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