Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)

Name: Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38734

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38734

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.063 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)

ELISA: Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric) [NBP3-38734]

ELISA: Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric) [NBP3-38734] - Standard Curve Reference

Kit Contents for Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Tripeptidyl-Peptidase I/TPP1

TPP1 encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Alternate Names

CLN2, LINCL, LPIC, TPP1, TripeptidylPeptidase I

Gene Symbol

TPP1

Additional Tripeptidyl-Peptidase I/TPP1 Products

Product Documents for Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Tripeptidyl-Peptidase I/TPP1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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