Rat UCP1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82465

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all UCP1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat UCP1 in samples. No Significant cross-reactivity or interference between Rat UCP1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.75

Inter-Assay Precision (Precision between assays) CV% < 5.48

Recovery for Rat UCP1 ELISA Kit (Colorimetric)

Recovery

91-107%

Linearity

Rat UCP1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat UCP1 ELISA Kit (Colorimetric)

Rat UCP1 ELISA Kit (Colorimetric)

ELISA: Rat UCP1 ELISA Kit (Colorimetric) [NBP2-82465] -

ELISA: Rat UCP1 ELISA Kit (Colorimetric) [NBP2-82465] - Standard Curve Reference

Kit Contents for Rat UCP1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: UCP1

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Gene Symbol

UCP1

Additional UCP1 Products

Product Documents for Rat UCP1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat UCP1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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