Human VCAM-1/CD106 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91177

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid (100 uL (1:50 prediluted)g)

Sensitivity

0.6 ng/mL

Assay Range

3.15 - 100 ng/mL

View all VCAM-1/CD106 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human VCAM-1/CD106. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human VCAM-1/CD106 positive serum.

Description

The human sVCAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sVCAM-1

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.10%

Inter-Assay Precision (Precision between assays) CV% < 5.20%

Recovery for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 85% to 104% with an overall mean recovery of 89%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

Human VCAM-1/CD106 ELISA Kit [NBP1-91177] - Standard curve for Human sVCAM-1 ELISA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VCAM-1/CD106

The vascular cell adhesion molecule-1 (VCAM-1) or CD106 is a member of the immunoglobulin gene superfamily. The initial molecular cloning of VCAM-1 reported six extracellular Ig-like domains (6D VCAM-1). This 6D VCAM-1 arises due to alternative splicing from a seven-domain VCAM-1 (7D VCAM-1). 7D VCAM-1 is the dominant form expressed by cultured human endothelial cells. Domains 1 through 3 are highly homologous to domains 4 through 6, suggesting that they arose by gene duplication. VCAM-1 is not constitutively expressed on endothelium, but can be upregulated in vitro in response to LPS, TNF-a, and IL-1, as well as to IFN-gamma and IL-4. VCAM-1 is also present on tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts and myotubes. A soluble form of VCAM-1 (sVCAM-1) has been described. Soluble VCAM-1 levels have been found in the serum of healthy individuals and increased levels of sVCAM-1 can be detected in several diseases. Cancer: ovarian, gastric-intestinal, renal, bladder cancer, non-Hodgkin's lymphoma, breast cancer (cyst fluid). Autoimmune diseases: multiple sclerosis (cerebrospinal fluid), systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis. Infections: sepsis, meningitis, malaria. Inflammation: vasculitis, alcoholic cirrhosis, primary biliary cirrhosis, Wegener's granulomatosis.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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