Rat VEGF-B ELISA Kit (Colorimetric)

Name: Rat VEGF-B ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-79679

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79679

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat VEGF-B in samples. No Significant cross-reactivity or interference between Rat VEGF-B and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.91%

Inter-Assay Precision (Precision between assays) CV% < 4.63%

Recovery for Rat VEGF-B ELISA Kit (Colorimetric)

Recovery

88-108%

Linearity

Scientific Data Images for Rat VEGF-B ELISA Kit (Colorimetric)

ELISA: Rat VEGF-B ELISA Kit (Colorimetric) [NBP2-79679] -

ELISA: Rat VEGF-B ELISA Kit (Colorimetric) [NBP2-79679] - Standard Curve Reference

Kit Contents for Rat VEGF-B ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGF-B

VEGFB 186 is an isoform of VEGFB (Vascular Endothelial Growth Factor B), a member of the vascular endothelial growth factor (VEGF) family. It is a growth factor for endothelial cells. Both isoforms of VEGFB show strong homology to VEGF at their amino termini, wheras the VEGFB167 isoform binds heparin, the C terminus of VEGFB-186 has no heparin binding domain, is less basic and more hydrophobic, and has no similarity to any published sequence. VEGFB 186 is secreted as a disulfide-linked homodimer and that, like VEGFB 167, this isoform can form disulfide-linked heterodimers with VEGF.

Long Name

Vascular Endothelial Growth Factor B

Alternate Names

VEGFB

Gene Symbol