Rat VEGF-C ELISA Kit (Colorimetric)

Name: Rat VEGF-C ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-92698

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92698

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (heparin) (50 uL)

Sensitivity

27.0 pg/mL

Assay Range

47.0 - 3,000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

The assay detects both natural and recombinant rat VEGF-C. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat VEGF-C positive serum. There was no cross reactivity or interference detected, notably not with rat VEGF-A, VEGF-B, VEGF-D, PDGF-AA,PDGF-BB, PLGF, HGF, EGF, VEGF-R1 and KDR.

Description

The rat VEGF-C ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat VEGF-C

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.1%

Inter-Assay Precision (Precision between assays) CV% < 2.7%

Scientific Data Images for Rat VEGF-C ELISA Kit (Colorimetric)

ELISA: VEGF-C ELISA Kit (Colorimetric) [NBP1-92698]

Kit Contents for Rat VEGF-C ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat VEGF-C
Biotin-Conjugate anti-rat VEGF-C monoclonal antibody
Conjugate Diluent
Rat VEGF-C Standard lyophilized, 6 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGF-C

Vascular endothelial growth factor-C (VEGF-C) is a member of the VEGF family of cytokines. It has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascular endothelial system. Two of its family members, VEGF-C and VEGF-D, regulate the lymphatic endothelial cells via their receptor VEGFR3, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C result in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Gene Symbol