Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Name: Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-83729

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83729

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL (prediluted 1:25))

Sensitivity

7.0 pg/mL

Assay Range

78 - 5000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human VEGFR2/KDR/Flk-1. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGFR2/KDR/Flk-1 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 at physiological concentrations and not with VEGF-R3/FLT-4. There was no interference detected, notably not for VEGF-A and VEGF-C.

Description

The human VEGF-R2/KDR ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R2/KDR.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.3%

Inter-Assay Precision (Precision between assays) CV% < 9.8%

Recovery for Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Recovery

Refer to manual

Scientific Data Images for Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

ELISA: Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) [NBP1-83729]

ELISA: VEGF R2/KDR/Flk-1 ELISA Kit [NBP1-83729] - Standard curve

Kit Contents for Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R2/KDR
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-human VEGF-R2/KDR monoclonal antibody
Human VEGF-R2/KDR Standard lyophilized, 10 ng/mL upon reconstitution
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGFR2/KDR/Flk-1

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

COA

SDS

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Product Specific Notices for Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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