Human Von Willebrand Factor ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0512

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Samples, Plasma, Serum (50 uL)

Sensitivity

0.38 mIU/mL

Assay Range

0.625 to 40 mIU/mL

View all Von Willebrand Factor ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

VWF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human VWF.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 7.0%

Recovery for Human Von Willebrand Factor ELISA Kit (Colorimetric)

Recovery

86-117%

Scientific Data Images for Human Von Willebrand Factor ELISA Kit (Colorimetric)

ELISA: Human Von Willebrand Factor ELISA Kit (Colorimetric) [KA0512]

ELISA: Human Von Willebrand Factor ELISA Kit (Colorimetric) [KA0512]

ELISA: Human Von Willebrand Factor ELISA Kit (Colorimetric) [KA0512] - Von Willebrand Factor ELISA Kit [KA0512] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Human Von Willebrand Factor ELISA Kit (Colorimetric)

  • Biotinylated Human VWF Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against VWF.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • Human VWF Microplate: A 96-well polystyrene microplate coated with a monoclonal antibody against VWF.
  • Human VWF Standard: Human VWF in a buffered protein base (lyophilized), calibrated against WHO 1st International Standard.
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Sealing Tapes: Precut, pressure sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Von Willebrand Factor

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.

Alternate Names

coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbol

VWF

Additional Von Willebrand Factor Products

Product Documents for Human Von Willebrand Factor ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Von Willebrand Factor ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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