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Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11045

Novus Biologicals, part of Bio-Techne
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NBP3-11045

Key Product Details

Sample Type & Volume Required Per Well

Cell Lysate, Tissue (100 uL)

Sensitivity

50 pg/mL

Assay Range

78.125 - 5000 pg/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Mouse, Rat

Recovery for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

Recovery

95.5%

Scientific Data Images for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve
ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.
ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045]

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

Kit Contents for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

  • 100X Poly HRP Concentrate - 150uL
  • 10X Wash Buffer Concentrate - 100mL
  • 5X VPS35 Extraction Reagent - 10mL
  • Anti-VPS35 Biotinylated Antibody Concentrate - 150uL
  • Anti-VPS35 Biotinylated Antibody Diluent (Green) - 13mL
  • Anti-VPS35 Immunoassay Plate - 1 plate
  • Recombinant VPS35 Standard - 2 vials
  • Standard and Sample Diluent (Red) - 50mL
  • Stop Solution - 13mL
  • Streptavidin: HRP Diluent (Purple) - 13mL
  • TMB Substrate - 13mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VPS35

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene Symbol

VPS35

Additional VPS35 Products

Product Documents for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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