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Human Wnt-5a ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38894

Novus Biologicals, part of Bio-Techne
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NBP3-38894

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

0.072 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Wnt-5a ELISA Kit (Colorimetric)

Human Wnt-5a ELISA Kit (Colorimetric)

ELISA: Human Wnt-5a ELISA Kit (Colorimetric) [NBP3-38894]

ELISA: Human Wnt-5a ELISA Kit (Colorimetric) [NBP3-38894] - Standard Curve Reference

Kit Contents for Human Wnt-5a ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

Additional Wnt-5a Products

Product Documents for Human Wnt-5a ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Wnt-5a ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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