Regulatory T Cell (Treg) Flow Cytometry Panel
R&D Systems, part of Bio-Techne | Catalog # FMC-P-004
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Multicolor flow cytometry panel to identify human T Regulatory Cells (Tregs). Human T regulatory cells were expanded from PBMCs using Anti-Human CD3 Monoclonal Antibody (10 μg/mL, MAB100) and Anti-human CD28 Monoclonal Antibody (5 μg/mL, AF-342-PB) plus Recombinant Human IL-2 (20 ng/mL, 202-IL), Recombinant Human TGF-beta (10 ng/mL, 77454-BH) for 2 days. Cells were stained with CD3 Alexa Fluor® 405, CD4 PerCP, CD25 PE, CD127 Alexa Fluor 700, FoxP3 Alexa Fluor 647, and GITR FITC. Find Staining Protocol Here. |
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