7-Aminoactinomycin D
Tocris Bioscience, part of Bio-Techne | Catalog # 7121
Key Product Details
| Description: | Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy |
| Chemical Name: | 2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide |
| Purity: | ≥97% (HPLC) |
| Molecular Weight: | 1270.45 |
Biological Activity for 7-Aminoactinomycin D
Key information: 7-aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain. It is membrane impermeant to live cells but penetrates dead or damaged cells.
Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.
Application: fluorescence microscopy and flow cytometry.
Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.
Optical Data for 7-Aminoactinomycin D
| λabs | 549 nm |
|---|---|
| λem | 655 nm |
| Application | Fluorescence Microscopy, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer