BDY FL Staurosporine
Tocris Bioscience, part of Bio-Techne | Catalog # 7985
Key Product Details
| Description: | Fluorescent probe targeting kinases; kinase tracer for TR-FRET assays |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 901.82 |
Biological Activity for BDY FL Staurosporine
BDY FL Staurosporine is a fluorescent kinase-targeting probe. It can be paired with antibodies labelled with TR-FRET donors such as CoraFluor™ 1 (Cat. No. 7920) for high throughput binding and protein quantification assays. BDY FL Staurosporine shows high specificity for tyrosine kinase (TK) and tyrosine kinase-like (TKL) family kinases. Excitation and emission maxima (λ) are 514 and 516 nm, respectively; quantum yield = 0.11; extinction coefficient = 35,000 M-1cm-1.
The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file (see below).
Supplementary Information
Optical Data for BDY FL Staurosporine
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 514 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 35400 M-1cm-1 |
| Quantum Yield (φ) | 0.11 |
| Closest Laser line | 488 nm |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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