CoraFluor™ 1, amine reactive
Tocris Bioscience, part of Bio-Techne | Catalog # 7920
Key Product Details
| Description: | Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation |
| Molecular Weight: | 1485.25 |
Biological Activity for CoraFluor™ 1, amine reactive
CoraFluor™ 1, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1, amine reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, thiol reactive and CoraFluor™ 2, amine reactive also available.
Please refer to the protocol for further information on conjugation.
CoraFluor™ is a trademark of Bio-Techne Corp.
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Optical Data for CoraFluor™ 1, amine reactive
| λabs | 338 nm |
|---|---|
| Extinction Coefficient (ε) | 23100 M-1cm-1 |
| Quantum Yield (φ) | 0.58 |
| Closest Laser line | 337 nm |
| Reactive Group | PFP ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.157 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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CoraFluor™ 1 stability profile. Stability profiling of CoraFluor™ 1 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM). |
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CoraFluor™ TR-FRET target engagement assay. High throughput TR-FRET target engagement assay profiling BRD4 inhibitors and Degraders on endogenous BRD4 using a single detection mix consisting of a primary BRD4 antibody, a CoraFluor™ 1 labeled VHH secondary antibody, and a fluorescent JQ1-based tracer (JQ1-FITC Catalog #7722). |
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