D-Luciferin sodium salt

Name: D-Luciferin sodium salt
Brand: Tocris Bioscience
SKU: 5427
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5427

(1)

Citations (8)

COA / SDS

Firefly luciferase substrate; cell permeable.

Key Product Details

Description:	Firefly luciferase substrate; cell permeable.
Chemical Name:	(4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
Purity:	≥99% (HPLC)
Molecular Weight:	302.3

View Full Technical Details for D-Luciferin sodium salt

Technical Data
Citations / Reviews (1)

Biological Activity for D-Luciferin sodium salt

D-Luciferin sodium salt is a firefly luciferase substrate (K_m = ~2 μM). Used in many standard bioluminescence imaging (BLI) techniques, including monitoring tumour growth, and investigating intracellular signaling activity in vitro and in vivo. Cell permeable. Emission maximum = 562 nm.

Optical Data for D-Luciferin sodium salt

Emission Color	Green
λ_em	562 nm
Cell Permeable	Yes
Application	Bioluminescence Imaging (BLI) Techniques, including monitoring tumor growth

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for D-Luciferin sodium salt

M. Wt	302.3
Formula	C₁₁H₇N₂NaO₃S₂
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	103404-75-7
PubChem ID	23710675
InChi Key	BZNVUYVALNTPBG-SREJTOIWSA-M
Smiles	OC1=CC2=C(N=C(C3=N[C@@H]([C@@]([O-])=O)CS3)S2)C=C1.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for D-Luciferin sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	30.23	100
	DMSO	30.23	100

Preparing Stock Solutions for D-Luciferin sodium salt

The following data is based on the product molecular weight 302.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.31 mL	16.54 mL	33.08 mL
5 mM	0.66 mL	3.31 mL	6.62 mL
10 mM	0.33 mL	1.65 mL	3.31 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

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References for D-Luciferin sodium salt

References are publications that support the biological activity of the product.

Keyaerts Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission. Eur.J.Nucl.Med.Mol.Imaging. 2008 PMID: 18180921