Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate
R&D Systems, part of Bio-Techne | Catalog # ES003
Substrate for TACE, ADAM9, and ADAM10
Key Product Details
Conjugate
7-Methoxycoumarin
Applications
Bioactivity
Product Specifications
Source
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2
Mca: (7-Methoxycoumarin-4-yl) acetyl, Dpa: N-3-(2, 4-Dinitrophenyl)-L-2,3-diaminopropionyl.
Purity
>95%, by HPLC.
Predicted Molecular Mass
1638 Da
Activity
The peptide substrate contains a highly fluorescent
7-methoxycoumarin group that is efficiently quenched by resonance energy
transfer to the 2,4-dinitrophenyl group. It can be used to measure the
activities of peptidases that are capable of cleaving an amide bond between the
fluorescent group and the quencher group, causing an increase in fluorescence.
It is an excellent substrate for tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between Ala and Val.
It is an excellent substrate for tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between Ala and Val.
Reviewed Applications
Read 1 review rated 5 using ES003 in the following applications:
Formulation, Preparation and Storage
ES003
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light. |
Background: Protease Substrates
Additional Protease Substrates Products
Product Documents for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate
Product Specific Notices for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate
For research use only
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