Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
R&D Systems, part of Bio-Techne | Catalog # ES005
Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin
Key Product Details
Conjugate
7-Methoxycoumarin
Applications
Binding Activity, Bioactivity
Product Specifications
Source
Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.
Purity
>95%, by HPLC.
Predicted Molecular Mass
1388 Da
Activity
The
peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is
efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl
group. It can be used to measure the activities of peptidases that are capable
of cleaving an amide bond between the fluorescent group and the quencher group,
causing an increase in fluorescence.
It is an excellent substrate for endothelin-converting enzyme-1 (ECE-1) and neprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe (Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286:112). The substrate is derived from bradykinin.
It is also an excellent substrate for ACE and active Cathepsin A and X/Z/P.
It is an excellent substrate for endothelin-converting enzyme-1 (ECE-1) and neprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe (Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286:112). The substrate is derived from bradykinin.
It is also an excellent substrate for ACE and active Cathepsin A and X/Z/P.
Formulation, Preparation and Storage
ES005
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light |
Background: Protease Substrates
Additional Protease Substrates Products
Product Documents for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
Product Specific Notices for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
For research use only
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