Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
R&D Systems, part of Bio-Techne | Catalog # ES004
Substrate for BACE-1
Key Product Details
Conjugate
7-Methoxycoumarin
Applications
Bioactivity
Product Specifications
Source
Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2
Mca: (7-Methoxycoumarin-4-yl) acetyl, Dnp: 2, 4-Dinitrophenyl
Predicted Molecular Mass
2001 Da
Activity
The
peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is
efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl
group. It can be used to measure the activities of peptidases that are capable
of cleaving an amide bond between the fluorescent group and the quencher group,
causing an increase in fluorescence.
The substrate contains the Swedish mutation of the amyloid precursor protein (APP) beta-secretase cleavage site. A K-R-R sequence is added for easy solubilization. It can be used to measure the activity of BACE-1 (R&D Systems, Catalog # 931-AS) and thimet oligopeptidase (Koike, H. et al., 1999, J. Biochem. 126:235).
The substrate contains the Swedish mutation of the amyloid precursor protein (APP) beta-secretase cleavage site. A K-R-R sequence is added for easy solubilization. It can be used to measure the activity of BACE-1 (R&D Systems, Catalog # 931-AS) and thimet oligopeptidase (Koike, H. et al., 1999, J. Biochem. 126:235).
Formulation, Preparation and Storage
ES004
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light |
Background: Protease Substrates
Additional Protease Substrates Products
Product Documents for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
Product Specific Notices for Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
For research use only
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