Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
R&D Systems, part of Bio-Techne | Catalog # ES007
Substrate for ACE-2 and Caspase-1
Key Product Details
Conjugate
7-Methoxycoumarin
Applications
Bioactivity
Product Specifications
Source
Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH.
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.
Purity
>95%, by HPLC.
Predicted Molecular Mass
1145.2 Da
Activity
The
peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is
efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl
group. It can be used to measure the activities of peptidases that are capable
of cleaving an amide bond between the fluorescent group and the quencher group,
causing an increase in fluorescence.
It is an excellent substrate for caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2) (Enari, M. et al., 1996, Nature 380:723; Vickers, C. et al., 2002, J. Biol. Chem. 277:14838). The cleavage sites by ICE and ACE-2 are the peptide bonds between Ala and Asp and between Pro and Lys, respectively.
It is an excellent substrate for caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2) (Enari, M. et al., 1996, Nature 380:723; Vickers, C. et al., 2002, J. Biol. Chem. 277:14838). The cleavage sites by ICE and ACE-2 are the peptide bonds between Ala and Asp and between Pro and Lys, respectively.
Reviewed Applications
Read 2 reviews rated 5 using ES007 in the following applications:
Formulation, Preparation and Storage
ES007
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light. |
Background: Protease Substrates
Additional Protease Substrates Products
Product Documents for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
Product Specific Notices for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
For research use only
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