Tocrifluor T1117

Name: Tocrifluor T1117
Brand: Tocris Bioscience
SKU: 2540

Tocris Bioscience, part of Bio-Techne | Catalog # 2540

Citations (5)

COA / SDS

Fluorescent cannabinoid ligand; fluorescent form of AM 251 (Cat. No. 1117)

Key Product Details

Description:	Fluorescent cannabinoid ligand; fluorescent form of AM 251 (Cat. No. 1117)
Chemical Name:	N-(Piperidin-1-yl)-5-(4-(4-(3-(5-carboxamidotetramethylrhodaminyl)propyl))phenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide
Purity:	≥95% (HPLC)
Molecular Weight:	974.97

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Technical Data
Citations

Biological Activity for Tocrifluor T1117

Tocrifluor T1117 is a fluorescent form of AM 251. AM 251 conjugated with 5-carboxytetramethylrhodamine (5-TAMRA) that fluoresces at 543 nm excitation (590 nm emission). Displays GPR55 binding activity. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

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Optical Data for Tocrifluor T1117

λ_abs	543 nm
λ_em	590 nm
Application	Fluorescence Microscopy, Confocal Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Tocrifluor T1117

M. Wt	974.97
Formula	C₅₆H₅₃Cl₂N₇O₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	1186195-59-4
PubChem ID	57369426
InChi Key	RNAPRXIZPJDYPH-UHFFFAOYSA-N
Smiles	CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)NCCCC1=CC=C(C=C1)C1=CC=C(C=C1)C1=C(C)C(=NN1C1=CC=C(Cl)C=C1Cl)C(=O)NN1CCCCC1)C1=C(O2)C=C(C=C1)N(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Tocrifluor T1117

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.75	10

Preparing Stock Solutions for Tocrifluor T1117

The following data is based on the product molecular weight 974.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	10.26 mL	51.28 mL	102.57 mL
0.5 mM	2.05 mL	10.26 mL	20.51 mL
1 mM	1.03 mL	5.13 mL	10.26 mL
5 mM	0.21 mL	1.03 mL	2.05 mL

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References for Tocrifluor T1117

References are publications that support the biological activity of the product.

Bruno Beyond radio-displacement techniques for identification of CB₁ ligands: the first application of a fluorescence-quenching assay. Sci.Rep. 2014 PMID: 24441508
Daly Visualisation of vascular cannabinoid receptors and their potential interaction with α₁-adrenergic receptors. Vascular and Smooth Muscle Phys.Soc. Symposium
Sylantyev Cannabinoid- and lysophosphatidylinositol-sensitive receptor GPR55 boosts neurotransmitter release at central synapses. Proc.Natl.Acad.Sci.U.S.A. 2013 PMID: 23472002
Daly Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteries. Br.J.Pharmacol. 2010 PMID: 20136833