Subcellular Protein Fractionation Kit
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47659
Key Product Details
Species
Mammal
Applications
Enzyme Activity, Gel Shift, Western Blot
Kit Type
Fractionation Kit
Product Summary for Subcellular Protein Fractionation Kit
The Cell Fractionation kit is designed to provide reproducible extraction of four subcellular protein fractions (cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
The percentage of proteins in the different fractions are cell-dependent, usually in the range of 20-30% cytosol, 25-35% membrane (organelles), 15-25% Nuclei, 15-25% insoluble.
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab288085)
The percentage of proteins in the different fractions are cell-dependent, usually in the range of 20-30% cytosol, 25-35% membrane (organelles), 15-25% Nuclei, 15-25% insoluble.
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab288085)
Product Specifications
Application Notes
In Western analysis, if a membrane protein appeares mainly in the nuclear fraction, the membrane extraction step may not be sufficient. Make sure to add Membrane Extraction Buffer B into the extraction, which contains NP-40 detergent. If it still shows the same result, increase incubation time from 1 min to 5 min after adding MEB-B. NP-40 should extract membrane proteins from most of cells.
Scientific Data Images for Subcellular Protein Fractionation Kit
Subcellular Protein Fractionation Kit [NBP2-47659]
Kit Contents for Subcellular Protein Fractionation Kit
- Cytosol Extraction Buffer (CEB)
- DTT (1 M)
- Membrane Extraction Buffer-A (MEB-A)
- Membrane Extraction Buffer-B (MEB-B)
- Nuclear Extraction Buffer (NEB)
- Protease Inhibitor Cocktail (lyophilized)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Subcellular Protein
Additional Subcellular Protein Products
Product Documents for Subcellular Protein Fractionation Kit
Product Specific Notices for Subcellular Protein Fractionation Kit
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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