Mouse IgG Fc Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96784
![Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784]](https://resources.bio-techne.com/images/products/Mouse IgG Fc Isotype Control-Western Blot-NBP1-96784-img0008.jpg "Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Mouse IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.
Host
Mouse
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c)
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Mouse IgG Fc Isotype Control
Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784]
Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.![SDS-PAGE: Mouse IgG Fc Isotype Control [NBP1-96784]](https://resources.bio-techne.com/images/products/Mouse-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96784-img0007.jpg "SDS-PAGE: Mouse IgG Fc Isotype Control [NBP1-96784]")
SDS-PAGE: Mouse IgG Fc Isotype Control [NBP1-96784]
SDS-Page: Mouse IgG Fc Isotype Control [NBP1-96784] - SDS-PAGE of Mouse IgG F(c) Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.Applications for Mouse IgG Fc Isotype Control
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Mouse IgG Fc Isotype Control
Product Specific Notices for Mouse IgG Fc Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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