Chicken IgG Fc Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96786
![SDS-PAGE: Chicken IgG Fc Isotype Control [NBP1-96786]](https://resources.bio-techne.com/images/products/Chicken-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96786-img0002.jpg "SDS-PAGE: Chicken IgG Fc Isotype Control [NBP1-96786]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Chicken
Applications
ELISA, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Chicken IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Chicken IgG F(ab')2 or anti-Papain.
Host
Chicken
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, anti-Chicken IgG and anti-Chicken IgG F(c)
Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Chicken IgG Fc Isotype Control
SDS-PAGE: Chicken IgG Fc Isotype Control [NBP1-96786]
SDS-Page: Chicken IgG Fc Isotype Control [NBP1-96786] - SDS-PAGE of Chicken IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.Applications for Chicken IgG Fc Isotype Control
Application
Recommended Usage
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Chicken IgG Fc Isotype Control
Product Specific Notices for Chicken IgG Fc Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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