Rat IgG Fc Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96967
![SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]](https://resources.bio-techne.com/images/products/Rat-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96967-img0003.jpg "SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Rat IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
Host
Rat
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c)
Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Rat IgG Fc Isotype Control
SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]
SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-PAGE of Rat IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.![SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]](https://resources.bio-techne.com/images/products/Rat-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96967-img0005.jpg "SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]")
SDS-PAGE: Rat IgG Fc Isotype Control [NBP1-96967]
SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.Applications for Rat IgG Fc Isotype Control
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rat IgG Fc Isotype Control
Product Specific Notices for Rat IgG Fc Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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