Rabbit IgG Fc Isotype Control [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97231
![SDS-PAGE: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231]](https://resources.bio-techne.com/images/products/Rabbit-IgG-Fc-Isotype-Control-%5BBiotin%5D-SDS-Page-NBP1-97231-img0001.jpg "SDS-PAGE: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rabbit
Applications
SDS-Page
Label
Biotin
Antibody Source
Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Rabbit IgG Fc Isotype Control [Biotin]
Specificity
No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain.
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum
Scientific Data Images for Rabbit IgG Fc Isotype Control [Biotin]
![SDS-PAGE: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231]](https://resources.bio-techne.com/images/products/Rabbit-IgG-Fc-Isotype-Control-[Biotin]-SDS-Page-NBP1-97231-img0001.jpg "SDS-PAGE: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231]")
SDS-PAGE: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231]
SDS-Page: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Biotin Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.Applications for Rabbit IgG Fc Isotype Control [Biotin]
Application
Recommended Usage
SDS-Page
1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit IgG Fc Isotype Control [Biotin]
Product Specific Notices for Rabbit IgG Fc Isotype Control [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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