Canine IgG Fc Isotype Control [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97250
Forumulation
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
HRP
Antibody Source
Canine IgG
Concentration
Please contact technical services for concentration.
Product Summary for Canine IgG Fc Isotype Control [HRP]
Specificity
No reaction was observed against anti-Dog IgG F(ab')2 or anti-Papain.
Host
Canine
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum
Applications for Canine IgG Fc Isotype Control [HRP]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Canine IgG Fc Isotype Control [HRP]
Product Specific Notices for Canine IgG Fc Isotype Control [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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