Armenian Hamster IgG Isotype Control (299Arm)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59616

Novus Biologicals, part of Bio-Techne
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NBP2-59616
NBP2-59616-500ug
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Key Product Details

Species Reactivity

Armenian Hamster

Applications

Control, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 299Arm

Concentration

0.5 mg/ml

View all IgG Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for Armenian Hamster IgG Isotype Control (299Arm)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500
Application Notes
This product is useful as an isotype control immunoglobulin This product has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody. .

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Armenian Hamster IgG Isotype Control (299Arm)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Armenian Hamster IgG Isotype Control (299Arm)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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