Rat IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97040
![Western Blot: Rat IgG Isotype Control [NBP1-97040]](https://resources.bio-techne.com/images/products/Rat-IgG-Isotype-Control-Western-Blot-NBP1-97040-img0002.jpg "Western Blot: Rat IgG Isotype Control [NBP1-97040]")
Forumulation
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Flow Cytometry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Rat IgG
Format
Purified
Concentration
LYOPH mg/ml
Product Specifications
Host
Rat
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rat IgG Isotype Control
Western Blot: Rat IgG Isotype Control [NBP1-97040]
Western Blot: Rat IgG Isotype Control [NBP1-97040] - SDS-Page: Rat IgG Isotype Control [Agarose Immobilized] [NBP1-97145] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Image using the Agarose form of this antibody.![SDS-PAGE: Rat IgG Isotype Control [NBP1-97040]](https://resources.bio-techne.com/images/products/Rat IgG Isotype Control-SDS-Page-NBP1-97040-img0003.jpg "SDS-PAGE: Rat IgG Isotype Control [NBP1-97040]")
SDS-PAGE: Rat IgG Isotype Control [NBP1-97040]
SDS-Page: Rat IgG Isotype Control [NBP1-97040] - SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 ug per lane.Applications for Rat IgG Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
SDS-Page
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and Western Blot and is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 500 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Rat IgG Isotype Control
Product Specific Notices for Rat IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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