Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, SDS-Page

Label

Agarose

Antibody Source

Mouse IgG

Concentration

Please contact technical services for concentration.

View all IgG Isotype Controls »

Product Specifications

Host

Mouse

Isotype

IgG

Description

Store vial at 4C prior to opening. DO NOT FREEZE.

This product is normal Mouse IgG coupled to activated agarose. Assay by immunoelectrophoresis prior to coupling to the beads.

Scientific Data Images for Mouse IgG Isotype Control [Agarose]

SDS-PAGE: Mouse IgG Isotype Control [Agarose] [NBP1-97142]

SDS-PAGE: Mouse IgG Isotype Control [Agarose] [NBP1-97142]

SDS-Page: Mouse IgG Isotype Control [Agarose] [NBP1-97142] - SDS-PAGE of Mouse IgG Whole Molecule Agarose Conjugated . Lane 1: 5 uL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule Agarose Conjugated . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa Myeloma Protein . Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Applications for Mouse IgG Isotype Control [Agarose]

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

SDS-Page

1:100 - 1:2000
Application Notes
This product is suitable for immunoprecipitation and Immunoaffinity purifications.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Mouse IgG Isotype Control [Agarose]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG Isotype Control [Agarose]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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