Chicken IgG Isotype Control [Alkaline Phosphatase]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97178

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Chicken

Applications

ELISA, Immunohistochemistry, SDS-Page, Western Blot

Label

Alkaline Phosphatase

Antibody Source

Chicken IgG

Format

Azide and BSA Free

Concentration

Please contact technical services for concentration.

View all IgG Isotype Controls »

Product Specifications

Host

Chicken

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken Serum and anti-Alkaline Phosphatase (calf intestine).

Scientific Data Images for Chicken IgG Isotype Control [Alkaline Phosphatase]

SDS-PAGE: Chicken IgG Isotype Control [Alkaline Phosphatase] [NBP1-97178]

SDS-PAGE: Chicken IgG Isotype Control [Alkaline Phosphatase] [NBP1-97178]

SDS-Page: Chicken IgG Isotype Control [Alkaline Phosphatase] [NBP1-97178] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Alkaline Phosphatase Conjugated. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane.

Applications for Chicken IgG Isotype Control [Alkaline Phosphatase]

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Immunohistochemistry

1:200 - 1:1000

SDS-Page

1:100 - 1:2000

Western Blot

1:500 - 1:2500
Application Notes
This product has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Azide and BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Chicken IgG Isotype Control [Alkaline Phosphatase]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Chicken IgG Isotype Control [Alkaline Phosphatase]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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