Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M2AK

Format

Purified

Concentration

Please contact technical services for concentration.

View all IgG2a Kappa Isotype Controls »

Product Specifications

Specificity

Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Mouse IgG2a Kappa Isotype Control (M2AK)

SDS-PAGE: Mouse IgG2a Kappa Isotype Control (M2AK) [NBP1-96981]

SDS-PAGE: Mouse IgG2a Kappa Isotype Control (M2AK) [NBP1-96981]

SDS-Page: Mouse IgG2a Kappa Isotype Control (M2AK) [NBP1-96981] - SDS-PAGE of Mouse IgG2a Kappa Isotype Control. Lane 1: Mouse IgG2a Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG2a Kappa Isotype Control, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 140 kDa Non-reduced, 55 and 25 Reduced.

Applications for Mouse IgG2a Kappa Isotype Control (M2AK)

Application
Recommended Usage

Flow Cytometry

1:1000-1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500
Application Notes
This product has been tested in SDS-Page, ELISA, and western blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Product Documents for Mouse IgG2a Kappa Isotype Control (M2AK)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG2a Kappa Isotype Control (M2AK)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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