Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27231AF488
Key Product Details
Species Reactivity
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Description
Scientific Data Images for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488]
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488] [NBP2-27231AF488]
Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488] [NBP2-27231AF488]
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 488] [NBP2-27231AF488] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488] [NBP2-27231AF488]
Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Applications for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488]
Flow Cytometry
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Formulation, Preparation, and Storage
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Formulation
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Stability & Storage
Background: IgG2B
Long Name
Additional IgG2B Products
Product Documents for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488]
Product Specific Notices for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 488]
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.