Mouse IgG3 Isotype Control (MG3) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96847
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG3 Clone # MG3
Concentration
LYOPH mg/ml
Product Summary for Mouse IgG3 Isotype Control (MG3) [FITC]
Specificity
Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product has been prepared from serum-free tissue culture supernate by protein A chromatography
This product has been prepared from serum-free tissue culture supernate by protein A chromatography
Scientific Data Images for Mouse IgG3 Isotype Control (MG3) [FITC]
Dot Blot: Mouse IgG3 Isotype Control (MG3) [FITC] [NBP1-96847] - Mouse IgG3 Protein [Fluorescein] [NBP1-96847] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose.
![Mouse IgG3 Isotype Control (MG3) [FITC]](https://resources.bio-techne.com/images/products/nbp1-96847_mouse-mouse-igg3-isotype-control-mg3-fitc-23520231753184.jpg "Mouse IgG3 Isotype Control (MG3) [FITC]")
Mouse IgG3 Isotype Control (MG3) [FITC]
Dot Blot showing the detection Fluorescein conjugated Mouse IgG3 subclass. A three-fold serial dilution of Mouse IgG3 Isotype Control (MG3) [FITC]starting at 200ng was spotted onto 0.45 ?m nitrocellulose and imaged using the Bio-Rad VersaDoc(R) 4000 MP.Applications for Mouse IgG3 Isotype Control (MG3) [FITC]
Application
Recommended Usage
Dot Blot
1:100 - 1:2000
ELISA
1:100 - 1:2000
Flow Cytometry
1:1000 - 1:5000
Fluorophore-linked immunosorbent assay
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG3
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene Symbol
IGHG3
Additional IgG3 Products
Product Documents for Mouse IgG3 Isotype Control (MG3) [FITC]
Product Specific Notices for Mouse IgG3 Isotype Control (MG3) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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