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Rat IgM Isotype Control [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97203

Novus Biologicals, part of Bio-Techne
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NBP1-97203

Key Product Details

Species Reactivity

Rat

Applications

SDS-Page

Label

HRP

Antibody Source

Rat IgM

Concentration

LYOPH mg/ml

Product Summary for Rat IgM Isotype Control [HRP]

Host

Rat

Isotype

IgM

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgM and anti-Rat Serum.

Scientific Data Images for Rat IgM Isotype Control [HRP]

SDS-PAGE: Rat IgM Isotype Control [HRP] [NBP1-97203]

SDS-PAGE: Rat IgM Isotype Control [HRP] [NBP1-97203]

SDS-Page: Rat IgM Isotype Control [HRP] [NBP1-97203] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Peroxidase Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.

Applications for Rat IgM Isotype Control [HRP]

Application
Recommended Usage

SDS-Page

1:100 - 1:2000
Application Notes
This product are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 500 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.04 M Potassium Phosphate, 0.30 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Rat IgM Isotype Control [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IgM Isotype Control [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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