Rat IgG2A Isotype Control Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB006
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Control
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 54447
Product Specifications
Immunogen
KLH
Specificity
Selected for use as rat IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta), rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhEotaxin-3, rmEotaxin 2, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin -1, rhLymphytactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1 delta, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 alpha, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTARC, rmTCA-3, rhTeck, or rmTeck.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat IgG2A Isotype Control
Detection of Rat IgG Control by Flow Cytometry
B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0105B).
Detection of Mouse IgG2a by Functional
CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant rat:mouse chimeric antibody having a mouse IgG1 Fc or (D) MAB529 (rat antibody) which is a commercial antibody that neutralizes CCL17. Assay was repeated twice with five replicates per sample each time. Data are expressed as chemotactic index (CI = Average RLU from stimulated wells/average RLU from medium control; medium control = CI of 1). Antibody dose used for each sample is indicated along the x axis. Composite CI results from both experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24339934), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Rat IgG2A Isotype Control
Application
Recommended Usage
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.6 using MAB006 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Rat IgG2A Isotype Control
Product Specific Notices for Rat IgG2A Isotype Control
For research use only
Loading...
Loading...
Loading...