Adenylate Cyclase 1 Overexpression Lysate

Name: Adenylate Cyclase 1 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-06564

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-06564

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Adenylate Cyclase 1 Overexpression Lysate

Adenylate Cyclase 1 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC222738

Accession#: NM_021116

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

123.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Adenylate Cyclase 1 Overexpression Lysate

Western Blot: Adenylate Cyclase 1 Overexpression Lysate [NBP2-06564]

Western Blot: Adenylate Cyclase 1 Overexpression Lysate (Adult Normal) [NBP2-06564] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenylate Cyclase 1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Adenylate Cyclase 1

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbetagamma and Galpha s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. AC I is expressed in myenteric ganglia as two bands of 160 kDa and 185 kDa by SDS-PAGE. Ca2+ stimulation of AC I and AC III is mediated by calmodulin. Protein associated with Myc (PAM) is a very potent inhibitor of AC I. A decrease in endogenous PAM levels in HeLa cells modulate both basal and agonist stimulated cAMP accumulation.

Long Name

Adenylate cyclase type 1

Alternate Names

ADCY1, Adenylyl cyclase 1, DFNB44, EC 4.6.1.1

Gene Symbol

ADCY1

Additional Adenylate Cyclase 1 Products

Product Documents for Adenylate Cyclase 1 Overexpression Lysate

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Adenylate Cyclase 1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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