Breast Matched Tumor and Normal Tissue Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47119
Key Product Details
Species
Human
Product Summary for Breast Matched Tumor and Normal Tissue Lysate
1 x Human breast tumor tissue lysate (1 mg/ml, 100 ug/vial)
1 x Human breast normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Infiltrating ductal carcinoma
Sex: Female
Age: 38
Grade: 1
Stage:
1 x Human breast normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Infiltrating ductal carcinoma
Sex: Female
Age: 38
Grade: 1
Stage:
IIIA, T3N1M0
Tumor Pathology Data
Location: Left breast
Gross findings: Tumor size 5 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined:
Microscopic Findings
The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells are arranged as groups which invaded the stroma. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. The stroma is infiltrated by small numbers of chronic inflammatory cells. Necroses are seen, but blood vessel invasions are not revealed. The lymph nodes show metastasis (4/13).
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
Product Specifications
Application Notes
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Type
Matched Tumor and Normal Tissue
Life Stage
Adult
Tissue
Breast
Formulation, Preparation, and Storage
Concentration
mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Breast
Additional Breast Products
Product Documents for Breast Matched Tumor and Normal Tissue Lysate
Product Specific Notices for Breast Matched Tumor and Normal Tissue Lysate
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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