COS-7 Nuclear Hypoxic Induced Cell Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NB800-PC26
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for COS-7 Nuclear Hypoxic Induced Cell Lysate
NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).
Product Specifications
Application Notes
Extracts have been quality control tested by Western blot. Before use mix 1:1 with 2X Sample Buffer and heat to 90C for 5 minutes before running on gel.
Specificity
NB800-PC26 contains hypoxia induced and uninduced COS-7 nuclear extract lysate
Type
Cell
Tissue
Digestive/Waste
Subcellular Fraction
Nuclear Hypoxic Induced
Scientific Data Images for COS-7 Nuclear Hypoxic Induced Cell Lysate
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]](https://resources.bio-techne.com/images/products/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]")
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]](https://resources.bio-techne.com/images/products/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]")
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]](https://resources.bio-techne.com/images/products/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]")
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).Formulation, Preparation, and Storage
Formulation
20 mM Hepes (pH 7.5), 400 mM NaCl, 0.1 mM EDTA, 10 mM NaF, 10 uM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors.
Preservative
20% glycerol
Concentration
2.5 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: COS-7
Additional COS-7 Products
Product Documents for COS-7 Nuclear Hypoxic Induced Cell Lysate
Product Specific Notices for COS-7 Nuclear Hypoxic Induced Cell Lysate
NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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