ERR beta/NR3B2 Overexpression Lysate

Name: ERR beta/NR3B2 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-04272

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04272

Submit a review

Product Datasheet / COA / SDS

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for ERR beta/NR3B2 Overexpression Lysate

ERR beta/NR3B2 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC215995

Accession#: NM_004452

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for ERR beta/NR3B2 Overexpression Lysate

Western Blot: ERR beta/NR3B2 Overexpression Lysate [NBP2-04272]

Western Blot: Estrogen Related Receptor beta Overexpression Lysate (Adult Normal) [NBP2-04272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Estrogen Related Receptor beta.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: ERR beta/NR3B2

Estrogen-related receptor beta (ERR beta) a NR3 Steroid Receptor, has been shown to affect early placental development. ERR beta also can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). ERR beta binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). ERR beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. ERR beta expression has been documented in mice in the extraembryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries.

Long Name

Estrogen-related Receptor beta

Alternate Names

ERR2, ESRL2, ESRRB, NR3B2

Gene Symbol

ESRRB

Additional ERR beta/NR3B2 Products

Product Documents for ERR beta/NR3B2 Overexpression Lysate

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ERR beta/NR3B2 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations
Reviews