Mouse Eye Tissue Lysate (Normal)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46608

Novus Biologicals, part of Bio-Techne
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NBP2-46608
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Key Product Details

Species

Mouse

Applications

Western Blot

Product Specifications

Specificity

Mouse whole eye tissue from balb/c mouse.

Type

Tissue

Tissue

Visual

Tissue Condition

Normal

Formulation, Preparation, and Storage

Formulation

SDS sample buffer containing 50 mM DTT.

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: Eye

Eye tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Additional Eye Products

Product Documents for Mouse Eye Tissue Lysate (Normal)

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Certificate of Analysis

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Product Specific Notices for Mouse Eye Tissue Lysate (Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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