Fucosyltransferase 3/FUT3 Overexpression Lysate

Name: Fucosyltransferase 3/FUT3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-11431

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11431

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Fucosyltransferase 3/FUT3 Overexpression Lysate

Fucosyltransferase 3/FUT3 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC215040

Accession#: NM_001097639

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Fucosyltransferase 3/FUT3 Overexpression Lysate

Western Blot: Fucosyltransferase 3/FUT3 Overexpression Lysate [NBP2-11431]

Western Blot: Lewis A Blood Group Antigen Overexpression Lysate (Native) [NBP2-11431] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Blood Group Lewis A.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Fucosyltransferase 3/FUT3

Because N-, O-glycans and glycolipids are frequently fucosylated at terminal sites, fucose is often found to be essential for sugar epitope and lectin ligand generation. Well-known fucose containing structures include Lewis structures and ABO blood group antigens. Lewis structures are key elements involved in leukocyte homing and extravasation process and thus are essential for lymphocyte maturation and natural defense functions. Fucose containing glycans also play essential roles in cell signaling and development. So far, more than 10 fucosyltransferases have been cloned from the human genome. FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis. FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are responsible for Lewis structure generation through their alpha 1-3 or alpha 1-4 fucosyltransferases activities. FUT3, also known as Lewis blood group fucosyltransferase, is unique by having both strong alpha 1-3 and alpha 1-4 fucosyltransferase activities. FUT3 has high homology with FUT5 and FUT6 due to gene duplication. FUT7 is exclusively responsible for biosynthesis of sialyl Lewis X epitope in leukocytes and high endothelial venule cells. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans. Predicted as type II transmembrane proteins and Golgi enzymes, some of the fucosyltransferases can also be found in plasma. R&D Systems rhFUTs correspond to the luminal domains.

Alternate Names

CD174, FT3B, FucT-III, FUT3, Les, Lewis FT

Gene Symbol

FUT3

Additional Fucosyltransferase 3/FUT3 Products

Product Documents for Fucosyltransferase 3/FUT3 Overexpression Lysate

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Fucosyltransferase 3/FUT3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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