Human Heart Whole Tissue Lysate (Adult Whole Normal)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10272

Novus Biologicals, part of Bio-Techne
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NBP2-10272-0.1mg
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Key Product Details

Species

Human

Applications

Western Blot

Product Specifications

Specificity

Human heart tissue

Type

Tissue

Life Stage

Adult

Tissue

Heart

Tissue Condition

Normal

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

SDS sample buffer containing 50 mM DTT.

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: Heart

Heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Additional Heart Products

Product Documents for Human Heart Whole Tissue Lysate (Adult Whole Normal)

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Certificate of Analysis

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Product Specific Notices for Human Heart Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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